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Anti-SMARCB1 Rabbit Monoclonal Antibody #2757

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Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using anti-SMARCB1 antibody (Cat#2757, 1:2,000). Green, isotype control; red, SMARCB1.
Immunocytochemical staining of HAP-1 cells with anti-SMARCB1 antibody (Cat#2757, 1:1,000) . Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    SMARCB1
  • 货号:
    2757
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-SMARCB1 Rabbit Monoclonal Antibody
Aliases SMARCB1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1; BAF47; PPP1R144; SNF5L1; INI-1; HSNFS; Sfh1p; Ini1; Snr1; SNF5; RDT; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1; Protein Phosphatase 1, Regulatory Subunit 144; Sucrose Nonfermenting, Yeast, Homolog-Like 1; Malignant Rhabdoid Tumor Suppressor; Integrase Interactor 1 Protein; BRG1-Associated Factor 47; SNF5 Homolog; HSNF5; SWI/SNF-Related Matrix-Associated Protein; Integrase Interactor 1; SWNTS1; MRD15; RTPS1; CSS3; INI1
Background

Gene Name: SMARCB1

NCBI Gene Entry: 6598

UniProt Entry: Q12824

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1935

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human SNF5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using anti-SMARCB1 antibody (Cat#2757, 1:2,000). Green, isotype control; red, SMARCB1.
  • Immunocytochemical staining of HAP-1 cells with anti-SMARCB1 antibody (Cat#2757, 1:1,000) . Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using anti-SMARCB1 antibody (Cat#2757, 1:2,000). Green, isotype control; red, SMARCB1.
Immunocytochemical staining of HAP-1 cells with anti-SMARCB1 antibody (Cat#2757, 1:1,000) . Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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