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Anti-Checkpoint Kinase 1 Mouse Monoclonal Antibody #2831

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Immunocytochemical staining of HepG2 cells with anti-GAPDH antibody (Cat#2835, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Checkpoint kinase 1 antibody (Cat#2831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CHEK1
  • 货号:
    2831
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Checkpoint Kinase 1 Mouse Monoclonal Antibody
Aliases CHEK1; Checkpoint Kinase 1; CHK1; Serine/Threonine-Protein Kinase Chk1; Cell Cycle Checkpoint Kinase; CHK1 Checkpoint Homolog; EC 2.7.11.1; Checkpoint, S. Pombe, Homolog Of, 1; CHK1 (Checkpoint, S.Pombe) Homolog; CHK1 Checkpoint Homolog (S. Pombe); Checkpoint Kinase-1; EC 2.7.11; OZEMA21; Chk1-S
Background

Gene Name: CHEK1

NCBI Gene Entry: 1111

UniProt Entry: O14757

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 54 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2745

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen Recombinant protein of human CHK1
Isotype Mouse IgM
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-GAPDH antibody (Cat#2835, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Checkpoint kinase 1 antibody (Cat#2831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HepG2 cells with anti-GAPDH antibody (Cat#2835, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Checkpoint kinase 1 antibody (Cat#2831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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