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Anti-HSPA5 Mouse Monoclonal Antibody #2840

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Immunocytochemical staining of HepG2 cells with anti-GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    HSPA5
  • 货号：
    2840
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-HSPA5 Mouse Monoclonal Antibody
Aliases HSPA5; Heat Shock Protein Family A (Hsp70) Member 5; BiP; GRP78; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Immunoglobulin Heavy Chain-Binding Protein; Heat Shock Protein 70 Family Protein 5; Heat Shock Protein Family A Member 5; Endoplasmic Reticulum Chaperone BiP; Glucose-Regulated Protein, 78kDa; 78 KDa Glucose-Regulated Protein; Binding-Immunoglobulin Protein; HSP70 Family Protein 5; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Epididymis Secretory Sperm Binding Protein Li 89n; EC 3.6.4.10; HEL-S-89n; GRP-78
Background

Gene Name: HSPA5

NCBI Gene Entry: 3309

UniProt Entry: P11021
Application Information

Molecular Weight: Predicted, 72 kDa; observed, 72 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2790

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen Recombinant protein of human GRP78
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    HSPA5
  • 货号：
    2840
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HepG2 cells with anti-GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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