Flow cytometric analysis of Keratin 18 expression in HepG2 cells using anti-Keratin 18 antibody (Cat#2847, 1:2,000). Green, isotype control; red, Keratin 18.
Immunocytochemical staining of HepG2 cells with anti-Keratin 18 antibody(Cat#2847, 1:1,000) . Nuclei were stained blue with DAPI; Keratin 18 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Keratin 18 antibody (Cat#2847). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 18 antibody (Cat#2847, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-glial fibrillary acidic protein antibody (Cat#2847, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
