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Anti-Keratin 6C Rabbit Monoclonal Antibody #2856

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Flow cytometric analysis of Keratin 6C expression in HepG2 cells using anti-Keratin 6C antibody (Cat#2856, 1:2,000). Green, isotype control; red, Keratin 6C.
Immunocytochemical staining of HepG2 cells with anti-Keratin 6C antibody(Cat#2856, 1:1000) . Nuclei were stained blue with DAPI; Keratin 6C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Keratin 6C antibody (Cat#2856). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 6C antibody (Cat#2856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • 基因名:
    KRT6C
  • 货号:
    2856
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Keratin 6C Rabbit Monoclonal Antibody
Aliases KRT6C; Keratin 6C; KRT6E; Keratin, Type II Cytoskeletal 6C; Type-II Keratin Kb12; Keratin 6C, Type II; Cytokeratin-6C; Cytokeratin-6E; Keratin K6h; Keratin 6E; CK-6C; CK-6E; K6C; Keratin-6C; PPKNEFD; K6E
Background

Gene Name: KRT6C

NCBI Gene Entry: 286887

UniProt Entry: P48668

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2860

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen Recombinant protein of human Keratin 6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Keratin 6C expression in HepG2 cells using anti-Keratin 6C antibody (Cat#2856, 1:2,000). Green, isotype control; red, Keratin 6C.
  • Immunocytochemical staining of HepG2 cells with anti-Keratin 6C antibody(Cat#2856, 1:1000) . Nuclei were stained blue with DAPI; Keratin 6C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Keratin 6C antibody (Cat#2856). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 6C antibody (Cat#2856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Keratin 6C expression in HepG2 cells using anti-Keratin 6C antibody (Cat#2856, 1:2,000). Green, isotype control; red, Keratin 6C.
Immunocytochemical staining of HepG2 cells with anti-Keratin 6C antibody(Cat#2856, 1:1000) . Nuclei were stained blue with DAPI; Keratin 6C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Keratin 6C antibody (Cat#2856). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 6C antibody (Cat#2856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
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