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Anti-CNOT4 Mouse Monoclonal Antibody #2868

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Immunocytochemical staining of HepG2 cells with anti-CNOT4 antibody(Cat#2868, 1:1,000) . Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CNOT4 antibody (Cat#2868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT4 antibody (Cat#2868, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CNOT4
  • 货号:
    2868
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CNOT4 Mouse Monoclonal Antibody
Aliases CNOT4;CCR4-NOT Transcription Complex Subunit 4; CLONE243; NOT4H; NOT4; Potential Transcriptional Repressor NOT4Hp; RING-Type E3 Ubiquitin Transferase CNOT4; E3 Ubiquitin-Protein Ligase CNOT4; CCR4-Associated Factor 4; NOT4 (Negative Regulator Of Transcription 4, Yeast) Homolog; CCR4-NOT Transcription Complex, Subunit 4; EC 2.3.2.27
Background

Gene Name: CNOT4

NCBI Gene Entry: 4850

UniProt Entry: O95628

Application Information

Molecular Weight: Predicted, 64 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2920

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen Recombinant protein of human CNOT4
Isotype Mouse IgG2a kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-CNOT4 antibody(Cat#2868, 1:1,000) . Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-CNOT4 antibody (Cat#2868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT4 antibody (Cat#2868, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HepG2 cells with anti-CNOT4 antibody(Cat#2868, 1:1,000) . Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CNOT4 antibody (Cat#2868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT4 antibody (Cat#2868, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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