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Anti-BCKDK Rabbit Monoclonal Antibody #2898

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Flow cytometric analysis of BCKDK expression in C2C12 cells using anti-BCKDK antibody (Cat#2898, 1:2,000). Green, isotype control; red, BCKDK
Immunocytochemical staining of C2C12 cells with anti-BCKDK antibody (Cat#2898, 1:1,000) . Nuclei were stained blue with DAPI; BCKDK was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-BCKDK antibody (Cat#2898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCKDK antibody (Cat#2898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    BCKDK
  • 货号:
    2898
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-BCKDK Rabbit Monoclonal Antibody
Aliases BCKDK; Branched Chain Keto Acid Dehydrogenase Kinase; [3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide]] Kinase, Mitochondrial; Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase; BCKDH Kinase; EC 2.7.11.4; BCKDHKIN; BDK; 3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide] Kinase, Mitochondrial; Branched Chain Alpha-Ketoacid Dehydrogenase Kinase; Branched Chain Ketoacid Dehydrogenase Kinase; BCKD-Kinase; EC 2.7.11.1; EC 2.7.11; BCKDKD
Background

Gene Name: BCKDK

NCBI Gene Entry: 10295

UniProt Entry: O14874

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3065

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human BCKDK
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of BCKDK expression in C2C12 cells using anti-BCKDK antibody (Cat#2898, 1:2,000). Green, isotype control; red, BCKDK
  • Immunocytochemical staining of C2C12 cells with anti-BCKDK antibody (Cat#2898, 1:1,000) . Nuclei were stained blue with DAPI; BCKDK was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-BCKDK antibody (Cat#2898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCKDK antibody (Cat#2898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of BCKDK expression in C2C12 cells using anti-BCKDK antibody (Cat#2898, 1:2,000). Green, isotype control; red, BCKDK
Immunocytochemical staining of C2C12 cells with anti-BCKDK antibody (Cat#2898, 1:1,000) . Nuclei were stained blue with DAPI; BCKDK was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-BCKDK antibody (Cat#2898). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCKDK antibody (Cat#2898, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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