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Anti-Chromobox Rabbit Monoclonal Antibody #2927

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Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#2927, 1:2,000). Green, isotype control; red, Chromobox 5.
Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#2927, 1:1,000) . Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Chromobox 5 antibody (Cat#2927). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromobox 5 antibody (Cat#2927, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CBX5
  • 货号：
    2927
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Chromobox Rabbit Monoclonal Antibody
Aliases CBX5; Chromobox ; HP1-ALPHA; HP1; Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila); Heterochromatin Protein 1 Homolog Alpha; Chromobox Protein Homolog 5; HP1Hs-Alpha; Antigen P25; HP1alpha; HP1A; Chromobox Homolog 5 (Drosophila HP1 Alpha); Heterochromatin Protein 1-Alpha; HP1 Alpha Homolog (Drosophila); Epididymis Luminal Protein 25; Chromobox Homolog 5; HP1 Alpha Homolog; HP1Hs Alpha; HP1 Alpha; HEL25
Background

Gene Name: CBX5

NCBI Gene Entry: 23468

UniProt Entry: P45973
Application Information

Molecular Weight: Predicted, 22 kDa; observed, 22 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3210

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human HP1 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#2927, 1:2,000). Green, isotype control; red, Chromobox 5.
  • Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#2927, 1:1,000) . Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Chromobox 5 antibody (Cat#2927). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromobox 5 antibody (Cat#2927, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CBX5
  • 货号：
    2927
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#2927, 1:2,000). Green, isotype control; red, Chromobox 5.
Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#2927, 1:1,000) . Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Chromobox 5 antibody (Cat#2927). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Chromobox 5 antibody (Cat#2927, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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