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Anti-FLI1 Rabbit Monoclonal Antibody #2929

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Flow cytometric analysis of FLI1 expression in HT-1080 cells using anti-FLI1 antibody (Cat#2929, 1:2,000). Green, isotype control; red, FLI1.

Immunocytochemical staining of HT-1080 cells with anti-FLI1 antibody (Cat#2929, 1:1,000) . Nuclei were stained blue with DAPI; FLI1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-FLI1 antibody (Cat#2929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLI1 antibody (Cat#2929, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FLI1
货号：

2929
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-FLI1 Rabbit Monoclonal Antibody
Aliases	FLI1; Fli-1 Proto-Oncogene, ETS Transcription Factor; SIC-; EWSR2; FLI-1; Friend Leukemia Integration 1 Transcription Factor; Friend Leukemia Virus Integration 1; Transcription Factor ERGB; Ewing Sarcoma Breakpoint Region 2; Proto-Oncogene Fli-1; BDPLT21
Background	Gene Name: FLI1 NCBI Gene Entry: 2313 UniProt Entry: Q01543
Application Information	Molecular Weight: Predicted, 51 kDa; observed, 51 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3220 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human FLI1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FLI1 expression in HT-1080 cells using anti-FLI1 antibody (Cat#2929, 1:2,000). Green, isotype control; red, FLI1.
Immunocytochemical staining of HT-1080 cells with anti-FLI1 antibody (Cat#2929, 1:1,000) . Nuclei were stained blue with DAPI; FLI1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-FLI1 antibody (Cat#2929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLI1 antibody (Cat#2929, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FLI1
货号：

2929
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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