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Anti-APC Rabbit Monoclonal Antibody #2938

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Flow cytometric analysis of APC expression in HepG2 cells using anti-APC antibody (Cat#2938, 1:2,000). Green, isotype control; red, APC.

Immunocytochemical staining of HepG2 cells with anti-APC antibody (Cat#2938, 1:1,000). Nuclei were stained blue with DAPI; APC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-APC antibody (Cat#2938). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APC antibody (Cat#2938, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

APC
货号：

2938
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-APC Rabbit Monoclonal Antibody
Aliases	APC; APC Regulator Of WNT Signaling Pathway; DP2.5; PPP1R46; DP2; DP3; Protein Phosphatase 1, Regulatory Subunit 46; APC, WNT Signaling Pathway Regulator; Adenomatous Polyposis Coli Protein; Deleted In Polyposis 2.5; Adenomatosis Polyposis Coli Tumor Suppressor; Epididymis Secretory Sperm Binding Protein; Adenomatous Polyposis Coli (APC); WNT Signaling Pathway Regulator; Adenomatosis Polyposis Coli; Adenomatous Polyposis Coli; Protein APC; BTPS2; DESMD; GS
Background	Gene Name: APC NCBI Gene Entry: 324 UniProt Entry: P25054
Application Information	Molecular Weight: Predicted, 312 kDa; observed, 160 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3260 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human APC
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of APC expression in HepG2 cells using anti-APC antibody (Cat#2938, 1:2,000). Green, isotype control; red, APC.
Immunocytochemical staining of HepG2 cells with anti-APC antibody (Cat#2938, 1:1,000). Nuclei were stained blue with DAPI; APC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-APC antibody (Cat#2938). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APC antibody (Cat#2938, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

APC
货号：

2938
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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