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Anti-Cell Division Cycle 45 Rabbit Monoclonal Antibody #2954

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Flow cytometric analysis of Cell division cycle 45 expression in HepG2 cells using anti-Cell division cycle 45 antibody (Cat#2954, 1:2,000). Green, isotype control; red, Cell division cycle 45.

Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 45 antibody (Cat#2954, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 45 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cell division cycle 45 antibody (Cat#2954). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cell division cycle 45 antibody (Cat#2954, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CDC45
货号：

2954
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Cell Division Cycle 45 Rabbit Monoclonal Antibody
Aliases	CDC45; Cell Division Cycle 45; CDC45L2; CDC45L; Cell Division Control Protein 45 Homolog; Human CDC45; PORC-PI-1; CDC45 (Cell Division Cycle 45, S.Cerevisiae, Homolog)-Like; CDC45 Cell Division Cycle 45-Like (S. Cerevisiae); Cell Division Cycle 45 Homolog (S. Cerevisiae); CDC45 Cell Division Cycle 45 Homolog; CDC45 Cell Division Cycle 45-Like; Cell Division Cycle 45 Homolog; Cell Division Cycle 45-Like 2; CDC45-Related Protein; MGORS7
Background	Gene Name: CDC45 NCBI Gene Entry: 8318 UniProt Entry: O75419
Application Information	Molecular Weight: Predicted, 66 kDa; observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3335 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CDC45
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Cell division cycle 45 expression in HepG2 cells using anti-Cell division cycle 45 antibody (Cat#2954, 1:2,000). Green, isotype control; red, Cell division cycle 45.
Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 45 antibody (Cat#2954, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 45 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cell division cycle 45 antibody (Cat#2954). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cell division cycle 45 antibody (Cat#2954, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CDC45
货号：

2954
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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