Anti-Cell Division Cycle 27 Rabbit Monoclonal Antibody - 产品中心

Anti-Cell Division Cycle 27 Rabbit Monoclonal Antibody #2955

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Flow cytometric analysis of Cell division cycle 27 expression in HepG2 cells using anti-Cell division cycle 27 antibody (Cat#2955, 1:2,000). Green, isotype control; red, Cell division cycle 27.

Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 27 antibody (Cat#2955, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cell division cycle 27 antibody (Cat#2955). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cell division cycle 27 antibody (Cat#2955, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CDC27
货号：

2955
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Cell Division Cycle 27 Rabbit Monoclonal Antibody
Aliases	CDC27; Cell Division Cycle 27; ANAPC3; APC3; D0S1430E; D17S978E; NUC2; Cell Division Cycle Protein 27 Homolog; Anaphase Promoting Complex Subunit 3; D0S1430E, D17S978E; CDC27Hs; H-NUC; Anaphase-Promoting Complex, Protein 3; Anaphase-Promoting Complex Subunit 3; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC
Background	Gene Name: CDC27 NCBI Gene Entry: 996 UniProt Entry: P30260
Application Information	Molecular Weight: Predicted, 92 kDa; observed, 91 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3340 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cdc27/APC3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Cell division cycle 27 expression in HepG2 cells using anti-Cell division cycle 27 antibody (Cat#2955, 1:2,000). Green, isotype control; red, Cell division cycle 27.
Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 27 antibody (Cat#2955, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cell division cycle 27 antibody (Cat#2955). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cell division cycle 27 antibody (Cat#2955, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CDC27
货号：

2955
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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