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Anti-DDAH1 Rabbit Monoclonal Antibody #2991

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Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
Immunocytochemical staining of HT-1080 cells with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:1,000) . Nuclei were stained blue with DAPI; Dimethylarginine dimethylaminohydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    DDAH1
  • 货号：
    2991
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-DDAH1 Rabbit Monoclonal Antibody
Aliases DDAH1; Dimethylarginine Dimethylaminohydrolase 1; DDAH; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase 1; Dimethylargininase-1; EC 3.5.3.18; DDAH-1; DDAHI; NG, NG-Dimethylarginine Dimethylaminohydrolase; Epididymis Secretory Protein Li 16; HEL-S-16
Background

Gene Name: DDAH1

NCBI Gene Entry: 23576

UniProt Entry: O94760
Application Information

Molecular Weight: Predicted, 31 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3520

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human DDAH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
  • Immunocytochemical staining of HT-1080 cells with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:1,000) . Nuclei were stained blue with DAPI; Dimethylarginine dimethylaminohydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    DDAH1
  • 货号：
    2991
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
Immunocytochemical staining of HT-1080 cells with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:1,000) . Nuclei were stained blue with DAPI; Dimethylarginine dimethylaminohydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#2991, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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