Anti-BUB3 Mitotic Checkpoint Protein Rabbit Monoclonal Antibody - 产品中心

Anti-BUB3 Mitotic Checkpoint Protein Rabbit Monoclonal Antibody #3024

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Flow cytometric analysis of BUB3 expression in HepG2 cells using anti-BUB3 antibody (Cat#3024, 1:2,000). Green, isotype control; red, BUB3.

Immunocytochemical staining of HepG2 cells with anti-BUB3 antibody (Cat#3024, 1:1,000) . Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-BUB3 antibody (Cat#3024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB3 antibody (Cat#3024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BUB3
货号：

3024
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-BUB3 Mitotic Checkpoint Protein Rabbit Monoclonal Antibody
Aliases	BUB3; BUB3 Mitotic Checkpoint Protein; BUB3L; Mitotic Checkpoint Protein BUB3; BUB3 (Budding Uninhibited By Benzimidazoles 3, Yeast) Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast); BUB3 Budding Uninhibited By Benzimidazoles 3 Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog; Testicular Tissue Protein Li 27; Budding Uninhibited By Benomyl; Mitotic Checkpoint Component; HBUB3
Background	Gene Name: BUB3 NCBI Gene Entry: 9184 UniProt Entry: O43684
Application Information	Molecular Weight: Predicted, 37 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3685 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Bub3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BUB3 expression in HepG2 cells using anti-BUB3 antibody (Cat#3024, 1:2,000). Green, isotype control; red, BUB3.
Immunocytochemical staining of HepG2 cells with anti-BUB3 antibody (Cat#3024, 1:1,000) . Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-BUB3 antibody (Cat#3024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB3 antibody (Cat#3024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BUB3
货号：

3024
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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