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Anti-EEF1A1 Rabbit Monoclonal Antibody #3042

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Flow cytometric analysis of Eukaryotic translation elongation factor 1 alpha 1 expression in HepG2 cells using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 1 alpha 1.
Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 1 alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    EEF1A1
  • 货号:
    3042
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-EEF1A1 Rabbit Monoclonal Antibody
Aliases EEF1A1; Eukaryotic Translation Elongation Factor 1 Alpha 1; Elongation Factor 1-Alpha 1; EF1alpha1; EE1A1; EF1A1; EEF1A; LENG7; EF1A; Leukocyte Receptor Cluster (LRC) Member 7; Leukocyte Receptor Cluster Member 7; Eukaryotic Elongation Factor 1 A-1; Elongation Factor Tu; EF-1-Alpha-1; EEF1A-1; EF-Tu; Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14; Glucocorticoid Receptor AF-1 Specific Elongation Factor; Translation Elongation Factor 1 Alpha 1-Like 14; Epididymis Secretory Sperm Binding Protein; Elongation Factor 1 Alpha Subunit; Prostate Tumor-Inducing Protein 1; Cervical Cancer Suppressor 3; CTCL Tumor Antigen; EF1a-Like Protein; EC 3.6.5.-; GRAF-1EF; CCS-3; EEF-1; CCS3; PTI1
Background

Gene Name: EEF1A1

NCBI Gene Entry: 1915

UniProt Entry: P68104

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3775

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human eEF1A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Eukaryotic translation elongation factor 1 alpha 1 expression in HepG2 cells using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 1 alpha 1.
  • Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 1 alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Eukaryotic translation elongation factor 1 alpha 1 expression in HepG2 cells using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 1 alpha 1.
Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 1 alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation elongation factor 1 alpha 1 antibody (Cat#3042, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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