Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E.
Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:1,000) . Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E.
Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:1,000) . Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E.
Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:1,000) . Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation initiation factor 4E antibody (Cat#3045, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
