Anti-Phospho-Nucleophosmin (S125) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-Nucleophosmin (S125) Rabbit Monoclonal Antibody #3123

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Flow cytometric analysis of phospho-nucleophosmin (S125) expression in HepG2 cells using anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:2,000). Green, isotype control; red, phospho-nucleophosmin (S125).

Immunocytochemical staining of HepG2 cells with anti-PKC antibody (Cat#3126, 1:1,000). Nuclei were stained blue with DAPI; PKC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-phospho-nucleophosmin (S125) antibody (Cat#3123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NPM1
货号：

3123
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Phospho-Nucleophosmin (S125) Rabbit Monoclonal Antibody
Aliases	NPM1; Nucleophosmin 1; NPM; Nucleophosmin; B23; Nucleophosmin (Nucleolar Phosphoprotein B23, Numatrin); Nucleophosmin/Nucleoplasmin Family, Member 1; Nucleolar Phosphoprotein B23; Nucleolar Protein NO38; Numatrin; Testicular Tissue Protein Li 128
Background	Gene Name: NPM1 NCBI Gene Entry: 4869 UniProt Entry: P06748
Application Information	Molecular Weight: Predicted, 33 kDa; observed, 35 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4180 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-Nucleophosmin (S125)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of phospho-nucleophosmin (S125) expression in HepG2 cells using anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:2,000). Green, isotype control; red, phospho-nucleophosmin (S125).
Immunocytochemical staining of HepG2 cells with anti-PKC antibody (Cat#3126, 1:1,000). Nuclei were stained blue with DAPI; PKC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-nucleophosmin (S125) antibody (Cat#3123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-nucleophosmin (S125) antibody (Cat#3123, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NPM1
货号：

3123
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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