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Anti-Sequestosome 1 Rabbit Monoclonal Antibody #3133

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Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#3133, 1:2,000). Green, isotype control; red, p62/SQSTM1.

Immunocytochemical staining of HepG2 cells with anti-p62/SQSTM1 antibody (Cat#3086, 1:1,000) . Nuclei were stained blue with DAPI; p62/SQSTM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#3133). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#3133, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SQSTM1
货号：

3133
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Sequestosome 1 Rabbit Monoclonal Antibody
Aliases	SQSTM1; Sequestosome 1; P60; P62B; A170; OSIL; P62; Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa; EBI3-Associated Protein Of 60 KDa; Oxidative Stress Induced Like; Ubiquitin-Binding Protein P62; Autophagy Receptor P62; Sequestosome-1; EBIAP; PDB3; Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62; EBI3-Associated Protein P60; Paget Disease Of Bone 3; FTDALS; NADGP; DMRV; ZIP3; ORCA
Background	Gene Name: SQSTM1 NCBI Gene Entry: 8878 UniProt Entry: Q13501
Application Information	Molecular Weight: Predicted, 48 kDa; observed, 62 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4225 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SQSTM1 / p62
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#3133, 1:2,000). Green, isotype control; red, p62/SQSTM1.
Immunocytochemical staining of HepG2 cells with anti-p62/SQSTM1 antibody (Cat#3086, 1:1,000) . Nuclei were stained blue with DAPI; p62/SQSTM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#3133). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#3133, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SQSTM1
货号：

3133
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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