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Anti-CRADD Rabbit Monoclonal Antibody #3134

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Flow cytometric analysis of CRADD expression in HepG2 cells using anti-CRADD antibody (Cat#3134, 1:2,000). Green, isotype control; red, CRADD.
Immunocytochemical staining of HepG2 cells with anti-CRADD antibody (Cat#3134, 1:1,000). Nuclei were stained blue with DAPI; CRADD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CRADD antibody (Cat#3134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRADD antibody (Cat#3134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CRADD
  • 货号:
    3134
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CRADD Rabbit Monoclonal Antibody
Aliases CRADD; CASP2 And RIPK1 Domain Containing Adaptor With Death Domain; RAIDD; RIP-Associated ICH1/CED3-Homologous Protein With Death Domain; Death Domain-Containing Protein CRADD; RIP-Associated Protein With A Death Domain; Caspase And RIP Adaptor With Death Domain; Caspase And RIP Adapter With Death Domain; Death Domain Containing Protein CRADD; Death Adaptor Molecule RAIDD; CRADD/LYZ Fusion; MRT34
Background

Gene Name: CRADD

NCBI Gene Entry: 8738

UniProt Entry: P78560

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4230

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human RAIDD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CRADD expression in HepG2 cells using anti-CRADD antibody (Cat#3134, 1:2,000). Green, isotype control; red, CRADD.
  • Immunocytochemical staining of HepG2 cells with anti-CRADD antibody (Cat#3134, 1:1,000). Nuclei were stained blue with DAPI; CRADD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-CRADD antibody (Cat#3134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRADD antibody (Cat#3134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CRADD expression in HepG2 cells using anti-CRADD antibody (Cat#3134, 1:2,000). Green, isotype control; red, CRADD.
Immunocytochemical staining of HepG2 cells with anti-CRADD antibody (Cat#3134, 1:1,000). Nuclei were stained blue with DAPI; CRADD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CRADD antibody (Cat#3134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRADD antibody (Cat#3134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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