Anti-Phospho-Chk1 (S280) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-Chk1 (S280) Rabbit Monoclonal Antibody #3136

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Flow cytometric analysis of phospho-chk1 (S280) expression in HeLa cells using anti-phospho-chk1 (S280) antibody (Cat#3136, 1:2,000). Green, isotype control; red, phospho-chk1 (S280).

Immunocytochemical staining of HeLa cells with anti-Phospho-Chk1 (S280) antibody (Cat#3136, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Chk1 (S280) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-phospho-chk1 (S280) antibody (Cat#3136). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-chk1 (S280) antibody (Cat#3136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CHEK1
货号：

3136
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Phospho-Chk1 (S280) Rabbit Monoclonal Antibody
Aliases	CHEK1; Checkpoint Kinase 1; CHK; Serine/Threonine-Protein Kinase Chk1; Cell Cycle Checkpoint Kinase; CHK1 Checkpoint Homolog; EC 2.7.11.1; Checkpoint, S. Pombe, Homolog Of, 1; CHK1 (Checkpoint, S.Pombe) Homolog; CHK1 Checkpoint Homolog (S. Pombe); Checkpoint Kinase-1; EC 2.7.11; OZEMA21; Chk1-S
Background	Gene Name: CHEK1 NCBI Gene Entry: 1111 UniProt Entry: O14757
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 54 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4240 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-Chk1 (S280)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of phospho-chk1 (S280) expression in HeLa cells using anti-phospho-chk1 (S280) antibody (Cat#3136, 1:2,000). Green, isotype control; red, phospho-chk1 (S280).
Immunocytochemical staining of HeLa cells with anti-Phospho-Chk1 (S280) antibody (Cat#3136, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Chk1 (S280) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-chk1 (S280) antibody (Cat#3136). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-chk1 (S280) antibody (Cat#3136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CHEK1
货号：

3136
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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