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Anti-Checkpoint Kinase 2 Rabbit Monoclonal Antibody #3225

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Flow cytometric analysis of Checkpoint kinase 2 expression in HepG2 cells using anti-Checkpoint kinase 2 antibody (Cat#3225, 1:2,000). Green, isotype control; red,Checkpoint kinase 2.

Immunocytochemical staining of HepG2 cells with anti-Checkpoint kinase 2 antibody(Cat#3225, 1:1,000). Nuclei were stained blue with DAPI; Checkpoint kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-checkpoint kinase 2 antibody (Cat#3225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-checkpoint kinase 2 antibody (Cat#3225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CHEK2
货号：

3225
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Checkpoint Kinase 2 Rabbit Monoclonal Antibody
Aliases	CHEK2; Checkpoint Kinase 2; CDS1; CHK2; PP1425; RAD53; Serine/Threonine-Protein Kinase Chk2; CHK2 Checkpoint Homolog; Cds1 Homolog; BA444G7; HuCds1; HCds1; CHK2 (Checkpoint, S.Pombe) Homolog; CHK2 Checkpoint Homolog (S. Pombe); Checkpoint-Like Protein CHK2; EC 2.7.11.1; EC 2.7.11 48; HUCDS1; Hucds1; LFS2
Background	Gene Name: CHEK2 NCBI Gene Entry: 11200 UniProt Entry: O96017
Application Information	Molecular Weight: Predicted, 61 kDa; observed, 61 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4590 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human Chk2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Checkpoint kinase 2 expression in HepG2 cells using anti-Checkpoint kinase 2 antibody (Cat#3225, 1:2,000). Green, isotype control; red,Checkpoint kinase 2.
Immunocytochemical staining of HepG2 cells with anti-Checkpoint kinase 2 antibody(Cat#3225, 1:1,000). Nuclei were stained blue with DAPI; Checkpoint kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-checkpoint kinase 2 antibody (Cat#3225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-checkpoint kinase 2 antibody (Cat#3225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CHEK2
货号：

3225
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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