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Anti-Actinin Alpha 3 Rabbit Monoclonal Antibody #3245

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Immunocytochemical staining of C2C12 cells with anti-Actinin alpha 3 antibody (Cat#3245, 1:1,000) . Nuclei were stained blue with DAPI; Actinin alpha 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Scale bar, 20 μm.

Western blotting analysis using anti-actinin alpha 3 antibody (Cat#3245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-actinin alpha 3 antibody (Cat#3245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ACTN3
货号：

3245
应用：

WB, IC
物种反应性：

M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Actinin Alpha 3 Rabbit Monoclonal Antibody
Aliases	ACTN3; Actinin Alpha 3; F-Actin Cross-Linking Protein; Alpha-Actinin-3; Alpha-Actinin Skeletal Muscle Isoform; Actinin Alpha 3 (Gene/Pseudogene); Alpha-Actinin Skeletal Muscle; Actinin, Alpha 3; ACTN3D
Background	Gene Name: ACTN3 NCBI Gene Entry: 89 UniProt Entry: Q08043
Application Information	Molecular Weight: Predicted, 103 kDa; observed, 103 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4680 Species Reactivity: Mouse Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ACTN3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of C2C12 cells with anti-Actinin alpha 3 antibody (Cat#3245, 1:1,000) . Nuclei were stained blue with DAPI; Actinin alpha 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-actinin alpha 3 antibody (Cat#3245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-actinin alpha 3 antibody (Cat#3245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ACTN3
货号：

3245
应用：

WB, IC
物种反应性：

M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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