Flow cytometric analysis of phospho-IRE1 (S724) expression in HT-1080 cells using anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:2,000). Green, isotype control; red, phospho-IRE1 (S724).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-IRE1 (S724) antibody (Cat#3345, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-IRE1 (S724) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-IRE1 (S724) antibody (Cat#3345). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of phospho-IRE1 (S724) expression in HT-1080 cells using anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:2,000). Green, isotype control; red, phospho-IRE1 (S724).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-IRE1 (S724) antibody (Cat#3345, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-IRE1 (S724) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-IRE1 (S724) antibody (Cat#3345). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of phospho-IRE1 (S724) expression in HT-1080 cells using anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:2,000). Green, isotype control; red, phospho-IRE1 (S724).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-IRE1 (S724) antibody (Cat#3345, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-IRE1 (S724) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-IRE1 (S724) antibody (Cat#3345). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-IRE1 (S724) antibody (Cat#3345, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
