Anti-P21 (RAC1) Activated Kinase 1 Rabbit Monoclonal Antibody - 产品中心

Anti-P21 (RAC1) Activated Kinase 1 Rabbit Monoclonal Antibody #3350

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Flow cytometric analysis of PAK1 expression in HepG2 cells using anti-PAK1 antibody (Cat#3350, 1:2,000). Green, isotype control; red, PAK1.

Immunocytochemical staining of HepG2 cells with anti-PAK1 antibody (Cat#3350, 1:1,000). Nuclei were stained blue with DAPI; PAK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-PAK1 antibody (Cat#3350). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAK1 antibody (Cat#3350, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PAK1
货号：

3350
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-P21 (RAC1) Activated Kinase 1 Rabbit Monoclonal Antibody
Aliases	PAK1; P21 (RAC1) Activated Kinase 1; P21/Cdc42/Rac1-Activated Kinase 1 (STE20 Homolog, Yeast); P21/Cdc42/Rac1-Activated Kinase 1 (Yeast Ste20-Related); P21 Protein (Cdc42/Rac)-Activated Kinase 1; Serine/Threonine-Protein Kinase PAK 1; STE20 Homolog, Yeast; EC 2.7.11.1; Alpha-PAK; P65-PAK; P21-Activated Kinase 1; EC 2.7.11; PAKalpha; IDDMSSD; PAK-1
Background	Gene Name: PAK1 NCBI Gene Entry: 5058 UniProt Entry: Q13153
Application Information	Molecular Weight: Predicted, 61 kDa; observed, 66 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5245 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PAK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PAK1 expression in HepG2 cells using anti-PAK1 antibody (Cat#3350, 1:2,000). Green, isotype control; red, PAK1.
Immunocytochemical staining of HepG2 cells with anti-PAK1 antibody (Cat#3350, 1:1,000). Nuclei were stained blue with DAPI; PAK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-PAK1 antibody (Cat#3350). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAK1 antibody (Cat#3350, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PAK1
货号：

3350
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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