Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HT-1080 cells using G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
Immunocytochemical staining of HT-1080 cells with G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:1,000) . Nuclei were stained blue with DAPI; G protein-coupled receptor kinase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#3646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HT-1080 cells using G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
Immunocytochemical staining of HT-1080 cells with G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:1,000) . Nuclei were stained blue with DAPI; G protein-coupled receptor kinase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#3646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HT-1080 cells using G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
Immunocytochemical staining of HT-1080 cells with G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:1,000) . Nuclei were stained blue with DAPI; G protein-coupled receptor kinase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#3646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#3646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
