Flow cytometric analysis of BRCA1 associated protein expression in H9c2 cells using anti-BRCA1 associated protein antibody (Cat#3654, 1:2,000). Green, isotype control; red, BRCA1 associated protein.
Immunocytochemical staining of H9C2 cells with anti-BRCA1 associated protein antibody (Cat#3654, 1:1,000) . Nuclei were stained blue with DAPI; BRCA1 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BRCA1 associated protein antibody (Cat#3654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BRCA1 associated protein antibody (Cat#3654, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen
Recombinant protein of human BRAP
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,500-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of BRCA1 associated protein expression in H9c2 cells using anti-BRCA1 associated protein antibody (Cat#3654, 1:2,000). Green, isotype control; red, BRCA1 associated protein.
Immunocytochemical staining of H9C2 cells with anti-BRCA1 associated protein antibody (Cat#3654, 1:1,000) . Nuclei were stained blue with DAPI; BRCA1 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BRCA1 associated protein antibody (Cat#3654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BRCA1 associated protein antibody (Cat#3654, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of BRCA1 associated protein expression in H9c2 cells using anti-BRCA1 associated protein antibody (Cat#3654, 1:2,000). Green, isotype control; red, BRCA1 associated protein.
Immunocytochemical staining of H9C2 cells with anti-BRCA1 associated protein antibody (Cat#3654, 1:1,000) . Nuclei were stained blue with DAPI; BRCA1 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BRCA1 associated protein antibody (Cat#3654). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BRCA1 associated protein antibody (Cat#3654, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
