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Anti-Ataxin 1 Rabbit Monoclonal Antibody #3675

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Flow cytometric analysis of Ataxin 1 expression in HT-1080 cells using anti-Ataxin 1 antibody (Cat#3675, 1:2,000). Green, isotype control; red, Ataxin 1.

Immunocytochemical staining of HT-1080 cells with anti-Ataxin 1 antibody (Cat#3675, 1:1,000) . Nuclei were stained blue with DAPI; Ataxin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-ataxin 1 antibody (Cat#3675). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ataxin 1 antibody (Cat#3675, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATXN1
货号：

3675
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Ataxin 1 Rabbit Monoclonal Antibody
Aliases	ATXN1; Ataxin 1; ATX1; D6S504E; SCA1; Spinocerebellar Ataxia Type 1 Protein; Ataxin-1; Spinocerebellar Ataxia 1 (Olivopontocerebellar Ataxia 1, Autosomal Dominant, Ataxin 1); Alternative Ataxin1
Background	Gene Name: ATXN1 NCBI Gene Entry: 6310 UniProt Entry: P54253
Application Information	Molecular Weight: Predicted, 87 kDa; observed, 110 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6850 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human Ataxin 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Ataxin 1 expression in HT-1080 cells using anti-Ataxin 1 antibody (Cat#3675, 1:2,000). Green, isotype control; red, Ataxin 1.
Immunocytochemical staining of HT-1080 cells with anti-Ataxin 1 antibody (Cat#3675, 1:1,000) . Nuclei were stained blue with DAPI; Ataxin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ataxin 1 antibody (Cat#3675). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ataxin 1 antibody (Cat#3675, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATXN1
货号：

3675
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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