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Anti-MutS Homolog 2 Mouse Monoclonal Antibody #3739

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Flow cytometric analysis of MSH2 expression in HAP-1 cells using anti-MSH2 antibody (Cat#3739, 1:2,000). Green, isotype control; red, MSH2.

Immunocytochemical staining of HAP-1 cells with anti-MSH2 antibody (Cat#3739, 1:1,000) . Nuclei were stained blue with DAPI; MSH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.

Western blotting analysis using anti-MSH2 antibody (Cat#3739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MSH2 antibody (Cat#3739, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MSH2
货号：

3739
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-MutS Homolog 2 Mouse Monoclonal Antibody
Aliases	MSH2; MutS Homolog 2; DNA Mismatch Repair Protein Msh2; HNPCC1; HNPCC; MSH-2; COCA1; HMSH2; MutS (E. Coli) Homolog 2 (Colon Cancer, Nonpolyposis Type 1); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1; DNA Mismatch Repair Protein Msh2 Transcript; MutS Protein Homolog 2; MutS-Like 2; LYNCH1; MMRCS2; LCFS2; FCC1
Background	Gene Name: MSH2 NCBI Gene Entry: 4436 UniProt Entry: P43246
Application Information	Molecular Weight: Predicted, 105 kDa; observed, 104 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB7150 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human MSH2
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of MSH2 expression in HAP-1 cells using anti-MSH2 antibody (Cat#3739, 1:2,000). Green, isotype control; red, MSH2.
Immunocytochemical staining of HAP-1 cells with anti-MSH2 antibody (Cat#3739, 1:1,000) . Nuclei were stained blue with DAPI; MSH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MSH2 antibody (Cat#3739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MSH2 antibody (Cat#3739, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MSH2
货号：

3739
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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