Flow cytometric analysis of Nucleoporin 50 expression in HAP-1 cells using anti-Nucleoporin 50 antibody (Cat#3759, 1:2,000). Green, isotype control; red, Nucleoporin 50.
Immunocytochemical staining of HAP1 cells with anti-Nucleoporin 50 antibody (Cat#3759, 1:1,000) . Nuclei were stained blue with DAPI;Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
Western blotting analysis using anti-nucleoporin 50 antibody (Cat#3759). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nucleoporin 50 antibody (Cat#3759, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Nucleoporin 50 expression in HAP-1 cells using anti-Nucleoporin 50 antibody (Cat#3759, 1:2,000). Green, isotype control; red, Nucleoporin 50.
Immunocytochemical staining of HAP1 cells with anti-Nucleoporin 50 antibody (Cat#3759, 1:1,000) . Nuclei were stained blue with DAPI;Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
Western blotting analysis using anti-nucleoporin 50 antibody (Cat#3759). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nucleoporin 50 antibody (Cat#3759, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Nucleoporin 50 expression in HAP-1 cells using anti-Nucleoporin 50 antibody (Cat#3759, 1:2,000). Green, isotype control; red, Nucleoporin 50.
Immunocytochemical staining of HAP1 cells with anti-Nucleoporin 50 antibody (Cat#3759, 1:1,000) . Nuclei were stained blue with DAPI;Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
Western blotting analysis using anti-nucleoporin 50 antibody (Cat#3759). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nucleoporin 50 antibody (Cat#3759, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
