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Anti-beta I Tubulin Rabbit Monoclonal Antibody #3789

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Western blotting analysis using anti-beta I Tubulin antibody (Cat#3789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta I Tubulin antibody (Cat#3789, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of beta I Tubulin expression in HAP-1 cells using anti-beta I Tubulin antibody (Cat#3789, 1:2,000). Green, isotype control; red, beta I Tubulin.
Immunocytochemical staining of HepG2 cells with anti-Beta I Tubulin protein antibody (Cat#3789, 1:1,000). Nuclei were stained blue with DAPI; Beta I Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. Permeabilization: Methanol.
  • 基因名:
    TUBB1
  • 货号:
    3789
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name Anti-beta I Tubulin Rabbit Monoclonal Antibody
Aliases TUBB1; Tubulin Beta 1 Class VI; Tubulin Beta-1 Chain; Tubulin, Beta 1; DJ543J19.4; Class VI Beta-Tubulin; MACTHC1
Background

Gene Name: TUBB1

NCBI Gene Entry: 81027

UniProt Entry: Q9H4B7

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB7880

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human beta I Tubulin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-beta I Tubulin antibody (Cat#3789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta I Tubulin antibody (Cat#3789, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of beta I Tubulin expression in HAP-1 cells using anti-beta I Tubulin antibody (Cat#3789, 1:2,000). Green, isotype control; red, beta I Tubulin.
  • Immunocytochemical staining of HepG2 cells with anti-Beta I Tubulin protein antibody (Cat#3789, 1:1,000). Nuclei were stained blue with DAPI; Beta I Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. Permeabilization: Methanol.
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Western blotting analysis using anti-beta I Tubulin antibody (Cat#3789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta I Tubulin antibody (Cat#3789, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of beta I Tubulin expression in HAP-1 cells using anti-beta I Tubulin antibody (Cat#3789, 1:2,000). Green, isotype control; red, beta I Tubulin.
Immunocytochemical staining of HepG2 cells with anti-Beta I Tubulin protein antibody (Cat#3789, 1:1,000). Nuclei were stained blue with DAPI; Beta I Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. Permeabilization: Methanol.
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