Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Green, isotype control; red, Cellular inhibitor of PP2A.
Immunocytochemical staining of HepG2 cells with anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:500) . Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
CIP2A; Cellular Inhibitor Of PP2A; KIAA1524; Cell Proliferation Regulating Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of Protein Phosphatase 2A; Cancerous Inhibitor Of PP2A; P90 Autoantigen; Protein CIP2A; NOCIVA; P90
Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen
Recombinant protein of human KIAA1524
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Green, isotype control; red, Cellular inhibitor of PP2A.
Immunocytochemical staining of HepG2 cells with anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:500) . Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Cellular inhibitor of PP2A expression in HepG2 cells using anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:1,000). Green, isotype control; red, Cellular inhibitor of PP2A.
Immunocytochemical staining of HepG2 cells with anti-Cellular inhibitor of PP2A antibody (Cat#3818, 1:500) . Nuclei were stained blue with DAPI; Cellular inhibitor of PP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-cellular inhibitor of PP2A antibody (Cat#3818). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cellular inhibitor of PP2A antibody (Cat#3818, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
