Flow cytometric analysis of CNOT2 expression in HAP-1 cells using anti-CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP1 cells with anti-CNOT2 antibody (Cat#3821, 1:500) . Nuclei were stained blue with DAPI; CNOT2stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNOT2 expression in HAP-1 cells using anti-CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP1 cells with anti-CNOT2 antibody (Cat#3821, 1:500) . Nuclei were stained blue with DAPI; CNOT2stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNOT2 expression in HAP-1 cells using anti-CNOT2 antibody (Cat#3821, 1:1,000). Green, isotype control; red, CNOT2.
Immunocytochemical staining of HAP1 cells with anti-CNOT2 antibody (Cat#3821, 1:500) . Nuclei were stained blue with DAPI; CNOT2stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CNOT2 antibody (Cat#3821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CNOT2 antibody (Cat#3821, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
