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Anti-CCT4 Mouse Monoclonal Antibody #3828

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Flow cytometric analysis of CCT4 expression in HepG2 cells using anti-CCT4 antibody (Cat#3828, 1:2,000). Green, isotype control; red, CCT4.

Immunocytochemical staining of HepG2 cells with anti-CCT4 antibody (Cat#3828, 1:500) . Nuclei were stained blue with DAPI; CCT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CCT4 antibody (Cat#3828). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT4 antibody (Cat#3828, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CCT4
货号：

3828
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Picture

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Information

Product Name	Anti-CCT4 Mouse Monoclonal Antibody
Aliases	CCT4; Chaperonin Containing TCP1 Subunit 4; Cctd; Chaperonin Containing TCP1, Subunit 4 (Delta); T-Complex Protein 1 Subunit Delta; Stimulator Of TAR RNA-Binding; TCP-1-Delta; SRB; Chaperonin Containing T-Complex Polypeptide 1, Delta Subunit; Chaperonin Containing T-Complex Polypeptide 1 Subunit 4; CCT-DELTA; CCT-Delta; CCTD
Background	Gene Name: CCT4 NCBI Gene Entry: 10575 UniProt Entry: P50991
Application Information	Molecular Weight: Predicted, 58 kDa; observed, 58 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB8075 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human CCT4
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CCT4 expression in HepG2 cells using anti-CCT4 antibody (Cat#3828, 1:2,000). Green, isotype control; red, CCT4.
Immunocytochemical staining of HepG2 cells with anti-CCT4 antibody (Cat#3828, 1:500) . Nuclei were stained blue with DAPI; CCT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CCT4 antibody (Cat#3828). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT4 antibody (Cat#3828, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CCT4
货号：

3828
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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