Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#3851, 1:1,000). Green, isotype control; red, Epsin 2.
Immunocytochemical staining of Hela cells with anti-Epsin 2 antibody (Cat#3851, 1:500) . Nuclei were stained blue with DAPI; Epsin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epsin 2 antibody (Cat#3851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#3851, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen
Recombinant protein of human EPN2
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#3851, 1:1,000). Green, isotype control; red, Epsin 2.
Immunocytochemical staining of Hela cells with anti-Epsin 2 antibody (Cat#3851, 1:500) . Nuclei were stained blue with DAPI; Epsin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epsin 2 antibody (Cat#3851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#3851, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#3851, 1:1,000). Green, isotype control; red, Epsin 2.
Immunocytochemical staining of Hela cells with anti-Epsin 2 antibody (Cat#3851, 1:500) . Nuclei were stained blue with DAPI; Epsin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epsin 2 antibody (Cat#3851). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#3851, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
