Flow cytometric analysis of Diphthamide biosynthesis 2 expression in HepG2 cells using anti-Diphthamide biosynthesis 2 antibody (Cat#3854, 1:1,000). Green, isotype control; red, Diphthamide biosynthesis 2.
Immunocytochemical staining of HepG2 cells with anti-Diphthamide biosynthesis 2 protein antibody (Cat#3854, 1:500) . Nuclei were stained blue with DAPI; Diphthamide biosynthesis 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-diphthamide biosynthesis 2 antibody (Cat#3854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-diphthamide biosynthesis 2 antibody (Cat#3854, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Diphthamide biosynthesis 2 expression in HepG2 cells using anti-Diphthamide biosynthesis 2 antibody (Cat#3854, 1:1,000). Green, isotype control; red, Diphthamide biosynthesis 2.
Immunocytochemical staining of HepG2 cells with anti-Diphthamide biosynthesis 2 protein antibody (Cat#3854, 1:500) . Nuclei were stained blue with DAPI; Diphthamide biosynthesis 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-diphthamide biosynthesis 2 antibody (Cat#3854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-diphthamide biosynthesis 2 antibody (Cat#3854, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Diphthamide biosynthesis 2 expression in HepG2 cells using anti-Diphthamide biosynthesis 2 antibody (Cat#3854, 1:1,000). Green, isotype control; red, Diphthamide biosynthesis 2.
Immunocytochemical staining of HepG2 cells with anti-Diphthamide biosynthesis 2 protein antibody (Cat#3854, 1:500) . Nuclei were stained blue with DAPI; Diphthamide biosynthesis 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-diphthamide biosynthesis 2 antibody (Cat#3854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-diphthamide biosynthesis 2 antibody (Cat#3854, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
