Flow cytometric analysis of Developmental pluripotency associated 4 expression in HAP-1 cells using anti-Developmental pluripotency associated 4 antibody (Cat#3858, 1:1,000). Green, isotype control; red, Developmental pluripotency associated 4.
Immunocytochemical staining of HAP1 cells with anti-Developmental pluripotency associated 4 antibody (Cat#3858, 1:500) . Nuclei were stained blue with DAPI; Developmental pluripotency associated 4 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-developmental pluripotency associated 4 antibody (Cat#3858). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-developmental pluripotency associated 4 antibody (Cat#3858, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
