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Anti-DVL2 Mouse Monoclonal Antibody #3866

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Flow cytometric analysis of Dishevelled segment polarity protein 2 expression in C2C12 cells using anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000). Green, isotype control; red, Dishevelled segment polarity protein 2.
Immunocytochemical staining of C2C12 cells with anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000) . Nuclei were stained blue with DAPI; Dishevelled segment polarity protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dishevelled segment polarity protein 2 antibody (Cat#3866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dishevelled segment polarity protein 2 antibody (Cat#3866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DVL2
  • 货号:
    3866
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-DVL2 Mouse Monoclonal Antibody
Aliases DVL2; Dishevelled Segment Polarity Protein 2; Segment Polarity Protein Dishevelled Homolog DVL-2; Dishevelled 2 (Homologous To Drosophila Dsh); Dishevelled, Dsh Homolog 2 (Drosophila); Dishevelled, Dsh Homolog 2; Dishevelled-2; DSH Homolog 2
Background

Gene Name: DVL2

NCBI Gene Entry: 1856

UniProt Entry: O14641

Application Information

Molecular Weight: Predicted, 79 kDa; observed, 79 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8260

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human DVL2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Dishevelled segment polarity protein 2 expression in C2C12 cells using anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000). Green, isotype control; red, Dishevelled segment polarity protein 2.
  • Immunocytochemical staining of C2C12 cells with anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000) . Nuclei were stained blue with DAPI; Dishevelled segment polarity protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-dishevelled segment polarity protein 2 antibody (Cat#3866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dishevelled segment polarity protein 2 antibody (Cat#3866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Dishevelled segment polarity protein 2 expression in C2C12 cells using anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000). Green, isotype control; red, Dishevelled segment polarity protein 2.
Immunocytochemical staining of C2C12 cells with anti-Dishevelled segment polarity protein 2 antibody (Cat#3866, 1:1,000) . Nuclei were stained blue with DAPI; Dishevelled segment polarity protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dishevelled segment polarity protein 2 antibody (Cat#3866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dishevelled segment polarity protein 2 antibody (Cat#3866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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