Flow cytometric analysis of Dedicator of cytokinesis 2 expression in HAP-1 cells using anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:1,000). Green, isotype control; red, Dedicator of cytokinesis 2.
Immunocytochemical staining of HAP1 cells with anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:500) . Nuclei were stained blue with DAPI; Dedicator of cytokinesis 2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-dedicator of cytokinesis 2 antibody (Cat#3888). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dedicator of cytokinesis 2 antibody (Cat#3888, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Dedicator of cytokinesis 2 expression in HAP-1 cells using anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:1,000). Green, isotype control; red, Dedicator of cytokinesis 2.
Immunocytochemical staining of HAP1 cells with anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:500) . Nuclei were stained blue with DAPI; Dedicator of cytokinesis 2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-dedicator of cytokinesis 2 antibody (Cat#3888). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dedicator of cytokinesis 2 antibody (Cat#3888, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Dedicator of cytokinesis 2 expression in HAP-1 cells using anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:1,000). Green, isotype control; red, Dedicator of cytokinesis 2.
Immunocytochemical staining of HAP1 cells with anti-Dedicator of cytokinesis 2 antibody (Cat#3888, 1:500) . Nuclei were stained blue with DAPI; Dedicator of cytokinesis 2 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-dedicator of cytokinesis 2 antibody (Cat#3888). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dedicator of cytokinesis 2 antibody (Cat#3888, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
