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Anti-Tubulin Beta 3 Class III Rabbit Monoclonal Antibody #3900

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Western blotting analysis using anti-beta Tubulin III antibody (Cat#3900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta Tubulin III antibody (Cat#3900, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of beta Tubulin III expression in HT-1080 cells using anti-beta Tubulin III antibody (Cat#3900, 1:2,000). Green, isotype control; red, beta Tubulin III.
Immunocytochemical staining of HT-1080 cells with anti-beta Tubulin III antibody (Cat#3900, 1:1,000). Nuclei were stained blue with DAPI; beta Tubulin III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • 基因名:
    TUBB3
  • 货号:
    3900
  • 应用:
    WB
  • 物种反应性:
    H, M
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name Anti-Tubulin Beta 3 Class III Rabbit Monoclonal Antibody
Aliases TUBB3; Tubulin Beta 3 Class III; CFEOM3A; Beta-4; CFEOM3; Class III Beta-Tubulin; Tubulin Beta-3 Chain; Tubulin Beta-4 Chain; Tubulin Beta-III; Tubulin, Beta 3; FEOM3; TUBB4; Fibrosis Of Extraocular Muscles, Congenital, 3; CDCBM1; CDCBM
Background

Gene Name: TUBB3

NCBI Gene Entry: 10381

UniProt Entry: Q13509

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8640

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human beta Tubulin III
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-beta Tubulin III antibody (Cat#3900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta Tubulin III antibody (Cat#3900, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of beta Tubulin III expression in HT-1080 cells using anti-beta Tubulin III antibody (Cat#3900, 1:2,000). Green, isotype control; red, beta Tubulin III.
  • Immunocytochemical staining of HT-1080 cells with anti-beta Tubulin III antibody (Cat#3900, 1:1,000). Nuclei were stained blue with DAPI; beta Tubulin III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Citation(0)
Western blotting analysis using anti-beta Tubulin III antibody (Cat#3900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta Tubulin III antibody (Cat#3900, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of beta Tubulin III expression in HT-1080 cells using anti-beta Tubulin III antibody (Cat#3900, 1:2,000). Green, isotype control; red, beta Tubulin III.
Immunocytochemical staining of HT-1080 cells with anti-beta Tubulin III antibody (Cat#3900, 1:1,000). Nuclei were stained blue with DAPI; beta Tubulin III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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