Anti-Prolyl 4-hydroxylase subunit beta Rabbit Monoclonal Antibody - 产品中心

Anti-Prolyl 4-hydroxylase subunit beta Rabbit Monoclonal Antibody #3922

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Flow cytometric analysis of Prolyl 4-hydroxylase subunit beta expression in C2C12 cells using anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:2,000). Green, isotype control; red, Prolyl 4-hydroxylase subunit beta.

Immunocytochemical staining of HepG2 cells with anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:1,000). Nuclei were stained blue with DAPI; Prolyl 4-hydroxylase subunit beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-prolyl 4-hydroxylase subunit beta antibody (Cat#3922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

P4HB
货号：

3922
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Picture

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Information

Product Name	Anti-Prolyl 4-hydroxylase subunit beta Rabbit Monoclonal Antibody
Aliases	Prolyl 4-Hydroxylase Subunit Beta; PDIA1; PDI; ERBA2L; PROHB; PO4HB; PO4DB; DSI; GIT; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide; Protein Disulfide Isomerase Family A, Member; Protein Disulfide Isomerase-Associated; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase, Beta Polypeptide; Collagen Prolyl 4-Hydroxylase Beta; Protein Disulfide-Isomerase; EC 5.3.4.1; P4Hbeta; P55; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase; Thyroid Hormone Binding Protein P55); Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase-Associated 1) Protein Disulfide Isomerase/Oxidoreductase; Glutathione-Insulin Transhydrogenase; Thyroid Hormone-Binding Protein P55; Testicular Secretory Protein Li 32; Protocollagen Hydroxylase; P4HBETA; CLCRP1; PHDB;
Background	Gene Name: P4HB NCBI Gene Entry: 5034 UniProt Entry: P07237
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 57 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB8700 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human P4HB
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Prolyl 4-hydroxylase subunit beta expression in C2C12 cells using anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:2,000). Green, isotype control; red, Prolyl 4-hydroxylase subunit beta.
Immunocytochemical staining of HepG2 cells with anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:1,000). Nuclei were stained blue with DAPI; Prolyl 4-hydroxylase subunit beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-prolyl 4-hydroxylase subunit beta antibody (Cat#3922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-prolyl 4-hydroxylase subunit beta antibody (Cat#3922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

P4HB
货号：

3922
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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