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Anti-CD58 Rabbit Monoclonal Antibody #3948

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Flow cytometric analysis of CD58 expression in HeLa cells using anti-CD58 antibody (Cat#3948, 1:2,000). Green, isotype control; red, CD58.

Immunocytochemical staining of Hela cells with anti-CD58 antibody (Cat#3948, 1:1,000) . Nuclei were stained blue with DAPI; CD58 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-CD58 antibody (Cat#3948). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD58 antibody (Cat#3948, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CD58
货号：

3948
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Citation(0)

Information

Product Name	Anti-CD58 Rabbit Monoclonal Antibody
Aliases	CD58; CD58 Molecule; LFA3; CD58 Antigen, (Lymphocyte Function-Associated Antigen 3); Lymphocyte Function-Associated Antigen 3; Surface Glycoprotein LFA-3; Ag3; CD58 Antigen; LFA-3
Background	Gene Name: CD58 NCBI Gene Entry: 965 UniProt Entry: P19256
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 37-75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB8830 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from CD58
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CD58 expression in HeLa cells using anti-CD58 antibody (Cat#3948, 1:2,000). Green, isotype control; red, CD58.
Immunocytochemical staining of Hela cells with anti-CD58 antibody (Cat#3948, 1:1,000) . Nuclei were stained blue with DAPI; CD58 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CD58 antibody (Cat#3948). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD58 antibody (Cat#3948, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CD58
货号：

3948
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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