Flow cytometric analysis of PEF1 expression in HeLa cells using anti-PEF1 antibody (Cat#3998, 1:2,000). Green, isotype control; red, PEF1.
Immunocytochemical staining of Hela cells with anti-PEF1 antibody (Cat#3998, 1:1,000). Nuclei were stained blue with DAPI; PEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PEF1 antibody (Cat#3998). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEF1 antibody (Cat#3998, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
PEF1; Penta-EF-Hand Domain Containing 1; Peflin; PEF1A; PEF Protein With A Long N-Terminal Hydrophobic Domain; ABP32; Epididymis Secretory Sperm Binding Protein; Penta-EF Hand Domain-Containing Protein 1
Flow cytometric analysis of PEF1 expression in HeLa cells using anti-PEF1 antibody (Cat#3998, 1:2,000). Green, isotype control; red, PEF1.
Immunocytochemical staining of Hela cells with anti-PEF1 antibody (Cat#3998, 1:1,000). Nuclei were stained blue with DAPI; PEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PEF1 antibody (Cat#3998). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEF1 antibody (Cat#3998, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PEF1 expression in HeLa cells using anti-PEF1 antibody (Cat#3998, 1:2,000). Green, isotype control; red, PEF1.
Immunocytochemical staining of Hela cells with anti-PEF1 antibody (Cat#3998, 1:1,000). Nuclei were stained blue with DAPI; PEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PEF1 antibody (Cat#3998). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEF1 antibody (Cat#3998, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
