Anti-Sonic Hedgehog Signaling Molecule Rabbit Monoclonal Antibody - 产品中心

Anti-Sonic Hedgehog Signaling Molecule Rabbit Monoclonal Antibody #4040

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Flow cytometric analysis of sonic hedgehog expression in HepG2 cells using anti-sonic hedgehog antibody (Cat#4040, 1:2,000). Green, isotype control; red, sonic hedgehog.

Immunocytochemical staining of HepG2 cells with anti-Sonic Hedgehog antibody (Cat#4040, 1:1,000). Nuclei were stained blue with DAPI; Sonic Hedgehog was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-sonic Hedgehog antibody (Cat#4040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sonic Hedgehog antibody (Cat#4040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

SHH
货号：

4040
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Sonic Hedgehog Signaling Molecule Rabbit Monoclonal Antibody
Aliases	SHH; Sonic Hedgehog Signaling Molecule; MCOPCB5; SMMCI; TPTPS; HHG1; TPT; Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains; Sonic Hedgehog Protein; ShhNC; HLP3; HPE3; Sonic Hedgehog (Drosophila) Homolog; Sonic Hedgehog Homolog; EC 3.1.-.-; HHG-1
Background	Gene Name: SHH NCBI Gene Entry: 6469 UniProt Entry: Q15465
Application Information	Molecular Weight: Predicted, 50 kDa; observed, 51 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9290 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Sonic Hedgehog
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of sonic hedgehog expression in HepG2 cells using anti-sonic hedgehog antibody (Cat#4040, 1:2,000). Green, isotype control; red, sonic hedgehog.
Immunocytochemical staining of HepG2 cells with anti-Sonic Hedgehog antibody (Cat#4040, 1:1,000). Nuclei were stained blue with DAPI; Sonic Hedgehog was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-sonic Hedgehog antibody (Cat#4040). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sonic Hedgehog antibody (Cat#4040, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

SHH
货号：

4040
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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