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Anti-ERK1 Rabbit Monoclonal Antibody #4044

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Flow cytometric analysis of ERK1 expression in HepG2 cells using anti-ERK1 antibody (Cat#4044, 1:2,000). Green, isotype control; red, ERK1.

Immunocytochemical staining of HepG2 cells with anti-ERK1 antibody (Cat#4044, 1:1,000) . Nuclei were stained blue with DAPI; ERK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ERK1 antibody (Cat#4044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1 antibody (Cat#4044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MAPK3
货号：

4044
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-ERK1 Rabbit Monoclonal Antibody
Aliases	MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase 3; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK
Background	Gene Name: MAPK3 NCBI Gene Entry: 5595 UniProt Entry: P27361
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9310 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ERK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ERK1 expression in HepG2 cells using anti-ERK1 antibody (Cat#4044, 1:2,000). Green, isotype control; red, ERK1.
Immunocytochemical staining of HepG2 cells with anti-ERK1 antibody (Cat#4044, 1:1,000) . Nuclei were stained blue with DAPI; ERK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ERK1 antibody (Cat#4044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1 antibody (Cat#4044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MAPK3
货号：

4044
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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