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Anti-EGFR Rabbit Monoclonal Antibody #4046

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Flow cytometric analysis of EGFR expression in HepG2 cells using anti-EGFR antibody (Cat#4046, 1:2,000). Green, isotype control; red, EGFR.

Immunocytochemical staining of HepG2 cells with anti-EGFR antibody(Cat#4046, 1:1,000). Nuclei were stained blue with DAPI;
EGFR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5.
Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-EGFR antibody (Cat#4046). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EGFR antibody (Cat#4046, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

EGFR
货号：

4046
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-EGFR Rabbit Monoclonal Antibody
Aliases	EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background	Gene Name: EGFR NCBI Gene Entry: 1956 UniProt Entry: P00533
Application Information	Molecular Weight: Predicted, 134 kDa; observed, 175 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9320 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human EGFR
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EGFR expression in HepG2 cells using anti-EGFR antibody (Cat#4046, 1:2,000). Green, isotype control; red, EGFR.
Immunocytochemical staining of HepG2 cells with anti-EGFR antibody(Cat#4046, 1:1,000). Nuclei were stained blue with DAPI;
EGFR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5.
Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-EGFR antibody (Cat#4046). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EGFR antibody (Cat#4046, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

EGFR
货号：

4046
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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