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Anti-Galectin 3 Rabbit Monoclonal Antibody #4052

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Flow cytometric analysis of Galectin 3 expression in C2C12 cells using anti-Galectin 3 antibody (Cat#4052, 1:2,000). Green, isotype control; red, Galectin 3.
Immunocytochemical staining of C2C12 cells with anti-galectin 3 antibody (Cat#4052, 1:1,000). Nuclei were stained blue with anti-DAPI; Galectin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-galectin 3 antibody (Cat#4052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galectin 3 antibody (Cat#4052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    LGALS3
  • 货号:
    4052
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Galectin 3 Rabbit Monoclonal Antibody
Aliases LGALS3; Galectin 3; GALIG; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein 3; IgE-Binding Protein 3; 35 KDa Lectin; Lectin L-29; Galectin-3; LGALS2; MAC-2; GALBP; MAC2; Epididymis Secretory Sperm Binding Protein 3; Galactoside-Binding Protein; MAC-2 Antigen; Mac-2 Antigen; CBP 35; CBP35; Gal-3; GAL3; L-31; L31
Background

Gene Name: LGALS3

NCBI Gene Entry: 3958

UniProt Entry: P17931

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9350

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Galectin 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Galectin 3 expression in C2C12 cells using anti-Galectin 3 antibody (Cat#4052, 1:2,000). Green, isotype control; red, Galectin 3.
  • Immunocytochemical staining of C2C12 cells with anti-galectin 3 antibody (Cat#4052, 1:1,000). Nuclei were stained blue with anti-DAPI; Galectin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-galectin 3 antibody (Cat#4052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galectin 3 antibody (Cat#4052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Galectin 3 expression in C2C12 cells using anti-Galectin 3 antibody (Cat#4052, 1:2,000). Green, isotype control; red, Galectin 3.
Immunocytochemical staining of C2C12 cells with anti-galectin 3 antibody (Cat#4052, 1:1,000). Nuclei were stained blue with anti-DAPI; Galectin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-galectin 3 antibody (Cat#4052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galectin 3 antibody (Cat#4052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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