Western blotting analysis using anti-vinculin antibody (Cat#4060). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vinculin antibody (Cat#4060, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Vinculin expression in HT-1080 cells using anti-Vinculin antibody (Cat#4060, 1:2,000). Green, isotype control; red, Vinculin.
Immunocytochemical staining of HT-1080 cells with anti-Vinculin antibody (Cat#4060, 1:1,000) . Nuclei were stained blue with DAPI; Vinculin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-vinculin antibody (Cat#4060). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vinculin antibody (Cat#4060, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Vinculin expression in HT-1080 cells using anti-Vinculin antibody (Cat#4060, 1:2,000). Green, isotype control; red, Vinculin.
Immunocytochemical staining of HT-1080 cells with anti-Vinculin antibody (Cat#4060, 1:1,000) . Nuclei were stained blue with DAPI; Vinculin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-vinculin antibody (Cat#4060). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vinculin antibody (Cat#4060, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Vinculin expression in HT-1080 cells using anti-Vinculin antibody (Cat#4060, 1:2,000). Green, isotype control; red, Vinculin.
Immunocytochemical staining of HT-1080 cells with anti-Vinculin antibody (Cat#4060, 1:1,000) . Nuclei were stained blue with DAPI; Vinculin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
