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Anti-PIK3C3 Rabbit Monoclonal Antibody #4098

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Flow cytometric analysis of PIK3C3 expression in HeLa cells using anti-PIK3C3 antibody (Cat#4098, 1:2,000). Green, isotype control; red, PIK3C3.
Immunocytochemical staining of HeLa cells with anti-PIK3C3 antibody (Cat#4098, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PIK3C3 antibody (Cat#4098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C3 antibody (Cat#4098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PIK3C3
  • 货号:
    4098
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PIK3C3 Rabbit Monoclonal Antibody
Aliases Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3; HVps34; Vps34; Phosphatidylinositol 3-Kinase P100 Subunit; Vacuolar Protein Sorting 34 Homolog; Phosphoinositide-3-Kinase, Class 3; PtdIns-3-Kinase Type 3; PI3-Kinase Type 3; EC 2.7.1.137; PI3K Type 3; Phosphatidylinositol 3-Kinase, Catalytic Subunit Type 3; Phosphoinositide-3-Kinase Class 3; EC 2.7.1; VPS34
Background

Gene Name: PIK3C3

NCBI Gene Entry: 5289

UniProt Entry: Q8NEB9

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9580

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PI 3 Kinase Class 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PIK3C3 expression in HeLa cells using anti-PIK3C3 antibody (Cat#4098, 1:2,000). Green, isotype control; red, PIK3C3.
  • Immunocytochemical staining of HeLa cells with anti-PIK3C3 antibody (Cat#4098, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-PIK3C3 antibody (Cat#4098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C3 antibody (Cat#4098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of PIK3C3 expression in HeLa cells using anti-PIK3C3 antibody (Cat#4098, 1:2,000). Green, isotype control; red, PIK3C3.
Immunocytochemical staining of HeLa cells with anti-PIK3C3 antibody (Cat#4098, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-PIK3C3 antibody (Cat#4098). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C3 antibody (Cat#4098, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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