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Anti-HIF 1 β Rabbit Monoclonal Antibody #4148

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Flow cytometric analysis of HIF-1 beta expression inHepG2 cells using anti-HIF-1beta antibody (Cat#4148, 1:2,000). Green, isotype control; red, HIF-1 beta.

Immunocytochemical staining of HepG2 cells with anti-HIF-1 beta antibody (Cat#4148, 1:1,000) . Nuclei were stained blue with DAPI; HIF-1 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-HIF-1 beta antibody (Cat#4148). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF-1 beta antibody (Cat#4148, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ARNT
货号：

4148
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-HIF 1 β Rabbit Monoclonal Antibody
Aliases	ARNT; Aryl Hydrocarbon Receptor Nuclear Translocator ; BHLHe2; HIF-1beta; Class E Basic Helix-Loop-Helix Protein 2; Hypoxia Inducible Factor 1; Subunit Beta; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; ARNT1; Hypoxia-Inducible Factor 1, Beta Subunit; Hypoxia-Inducible Factor 1-Beta; ARNT Protein; HIF1BETA; BHLHE2; HIF1B; TANGO
Background	Gene Name: ARNT NCBI Gene Entry: 405 UniProt Entry: P27540
Application Information	Molecular Weight: Predicted, 87 kDa; observed, 87 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9800 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HIF-1 beta
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of HIF-1 beta expression inHepG2 cells using anti-HIF-1beta antibody (Cat#4148, 1:2,000). Green, isotype control; red, HIF-1 beta.
Immunocytochemical staining of HepG2 cells with anti-HIF-1 beta antibody (Cat#4148, 1:1,000) . Nuclei were stained blue with DAPI; HIF-1 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HIF-1 beta antibody (Cat#4148). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF-1 beta antibody (Cat#4148, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ARNT
货号：

4148
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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