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Anti-PTPN11 Rabbit Monoclonal Antibody #4204

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Flow cytometric analysis of PTPN11 expression in HepG2 cells using anti-PTPN11 antibody (Cat#4204, 1:2,000). Green, isotype control; red, PTPN11.

Immunocytochemical staining of HepG2 cells with anti-PTPN11 antibody (Cat#4204, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PTPN11 antibody (Cat#4204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#4204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PTPN11
货号：

4204
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-PTPN11 Rabbit Monoclonal Antibody
Aliases	PTPN11; Protein Tyrosine Phosphatase Non-Receptor Type 11; SH-PTP2; PTP2C; BPTP3; SHP-2; SHP2; SH2 Domain-Containing Protein Tyrosine Phosphatase 2; Tyrosine-Protein Phosphatase Non-Receptor Type 11; Protein-Tyrosine Phosphatase 1D; Protein-Tyrosine Phosphatase 2C; EC 3.1.3.48; SH-PTP3; PTP-1D; NS1; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Noonan Syndrome 1; METCDS; SHPTP2; PTP-2C; JMML; Shp2; CFC
Background	Gene Name: PTPN11 NCBI Gene Entry: 5781 UniProt Entry: Q06124
Application Information	Molecular Weight: Predicted, 68 kDa; observed, 68 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10055 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SHP2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PTPN11 expression in HepG2 cells using anti-PTPN11 antibody (Cat#4204, 1:2,000). Green, isotype control; red, PTPN11.
Immunocytochemical staining of HepG2 cells with anti-PTPN11 antibody (Cat#4204, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PTPN11 antibody (Cat#4204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#4204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PTPN11
货号：

4204
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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